Document Details

Document Type : Article In Journal 
Document Title :
A comparative study of first-derivative spectrophotometry and column high-performance liquid chromatography applied to the determination of repaglinide in tablets and for dissolution testing
دراسة مقارنة الطيف المشتق الأول والكروماتوغرافيا عالية الأداء لتحديد repaglinide في أقراص الرباغلنايد لاختبار التركيز وانحلال الدواء
 
Subject : Pharmaceutical Analytical Chemistry 
Document Language : English 
Abstract : A fast and reliable method for the determination of repaglinide is highly desirable to support formulation screening and quality control. A first-derivative UV spectroscopic method was developed for the determination of repaglinide in tablet dosage form and for dissolution testing. First-derivative UV absorbance was measured at 253 nm. The developed method was validated for linearity, accuracy, precision, limit of detection (LOD), and limit of quantitation (LOQ) in comparison to the U.S. Pharmacopeia (USP) column high-performance liquid chromatographic (HPLC) method. The first-derivative UV spectrophotometric method showed excellent linearity [correlation coefficient (r) = 0.9999] in the concentration range of 1-35 μg/mL and precision (relative standard deviation <1.5%). The LOD and LOQ were 0.23 and 0.72 μg/mL, respectively, and good recoveries were achieved (98-101.8%). Statistical comparison of results of the first-derivative UV spectrophotometric and the USP HPLC methods using the Mest showed that there was no significant difference between the 2 methods. Additionally, the method was successfully used for the dissolution test of repaglinide and was found to be reliable, simple, fast, and inexpensive. 
ISSN : 10603271 
Journal Name : Journal of AOAC International 
Volume : 91 
Issue Number : 3 
Publishing Year : 1429 AH
2008 AD 
Article Type : Article 
Added Date : Wednesday, December 22, 2010 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
بشار الخالدي ماجد شتيوي ياسر البستنجيBustanji, Y, AlKhalidi, B Shtaiwi, MResearcherDoctoratemajedshtaiwi@yahoo.co.uk

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