Document Details

Document Type : Article In Journal 
Document Title :
High-throughput genotyping assay for the large-scale genetic characterization of Cryptosporidium parasites from human and bovine samples.
High-throughput genotyping assay for the large-scale genetic characterization of Cryptosporidium parasites from human and bovine samples.
 
Document Language : English 
Abstract : The epidemiological study of human cryptosporidiosis requires the characterization of species and subtypes involved in human disease in large sample collections. Molecular genotyping is costly and time-consuming, making the implementation of low-cost, highly efficient technologies increasingly necessary. Here, we designed a protocol based on MALDI-TOF mass spectrometry for the high-throughput genotyping of a panel of 55 single nucleotide variants (SNVs) selected as markers for the identification of common gp60 subtypes of four Cryptosporidium species that infect humans. The method was applied to a panel of 608 human and 63 bovine isolates and the results were compared with control samples typed by Sanger sequencing. The method allowed the identification of species in 610 specimens (90·9%) and gp60 subtype in 605 (90·2%). It displayed excellent performance, with sensitivity and specificity values of 87·3 and 98·0%, respectively. Up to nine genotypes from four different Cryptosporidium species (C. hominis, C. parvum, C. meleagridis and C. felis) were detected in humans; the most common ones were C. hominis subtype Ib, and C. parvum IIa (61·3 and 28·3%, respectively). 96·5% of the bovine samples were typed as IIa. The method performs as well as the widely used Sanger sequencing and is more cost-effective and less time consuming. 
ISSN : 1469-8161 
Journal Name : Parasitology 
Volume : 141 
Issue Number : 1 
Publishing Year : 1434 AH
2013 AD 
Article Type : Article 
Added Date : Thursday, April 14, 2016 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
JL Abal-FabeiroAbal-Fabeiro, JL Researcher  
X MasideMaside, X Researcher  
J LlovoLlovo, J Researcher  
X BelloBello, X Researcher  
M TorresTorres, M Researcher  
M TreviñoTreviño, M Researcher  
L MoldesMoldes, L Researcher  
A MuñozMuñoz, A Researcher  
A CarracedoCarracedo, A Researcher  
C BartoloméBartolomé, C Investigator  

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