Document Details

Document Type : Article In Journal 
Document Title :
Spectrofluorimetric method for measuring the activity of the enzyme α-l-fucosidase using the ion associate of 2-chloro-4-nitro phenol-rhodamine-B
Spectrofluorimetric method for measuring the activity of the enzyme α-l-fucosidase using the ion associate of 2-chloro-4-nitro phenol-rhodamine-B
 
Subject : Chemistry 
Document Language : English 
Abstract : A low cost and accurate method for the detection and analytical determination of the activity of the enzyme α-l-fucosidase (AFU) was developed. The method was based upon measuring the fluorescence intensity of the complex ion associate of the ion associate of rhodamine-B and the compound 2-chloro-4-nitrophenol (RB+ CNP-) at 580 nm in phosphate buffer (pH 5) against the reagent blank. The influence of the different parameters, e.g. pH, incubation time, temperature, 2-chloro-4-nitrophenol concentration, foreign ions and surfactants that control the fluorescence intensity of the produced ion associate was critically investigated. The correlation between the fluorescence activity of the enzyme AFU by the developed procedures and the standard method was positive and highly significant in patients and controls (r2 = 0.99, p < 0.001). The developed method is simple and proceeds without practical artifacts compared to the standard method. 
ISSN : 0 
Journal Name : Talanta 
Volume : 80 
Issue Number : 1 
Publishing Year : 2009 AH
2009 AD 
Number Of Pages : 5 
Article Type : Article 
Added Date : Monday, October 5, 2009 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
محمدسرور السعيد شحاته الشهاويEl-Shahawi, M.SResearcherDoctorateMALSAEED@kau.edu.sa
-Othman, A.M.ResearcherDoctorate 
-El-Houseini, M.EResearcherDoctorate 
-Nashed, B.ResearcherDoctorate 
-Elsofy, M.SResearcherDoctorate 

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