Document Details

Document Type : Article In Journal 
Document Title :
Synthesis and characterization of new chromogenic substrates for exoglycosidases: alpha-glucosidase, alpha-mannosidase, and beta-galactosidase
Synthesis and characterization of new chromogenic substrates for exoglycosidases: alpha-glucosidase, alpha-mannosidase, and beta-galactosidase
 
Document Language : English 
Abstract : Glycosides of 4-nitrocatechol (1,2-dihydroxy 4-nitrobenzene) with alpha-D-glucopyranose and alpha-D-mannopyranose were synthesized by the glycosylation of phenol with peracetylated sugars in the presence of BF3 center dot OBu2. The glycoside of 4-nitrocatechol with beta-D-galactopyranose was prepared by the glycosylation of this phenol as sodium phenoxide with tetra-O-benzoyl-alpha-D-galactopyranosyl bromide. The structure of the reaction products was confirmed by H-1 and C-13 NMR spectra and by chemical analysis. The latter consisted of acidic hydrolysis, followed by ethyl ether extraction and colorimetric determination of 4-nitrocatechol in the ether phase and application of the anthrone method for the sugar in the water phase. The synthetic glycosides were tested as substrates for enzymes from animal, vegetal, and microbial materials. 
ISSN : 1300-0527 
Journal Name : TURKISH JOURNAL OF CHEMISTRY 
Volume : 37 
Issue Number : 2 
Publishing Year : 1434 AH
2013 AD 
Article Type : Article 
Added Date : Sunday, July 23, 2017 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
D IgaIga, D Researcher  
R SchmidtSchmidt, R Researcher  
S IgaIga, S Researcher  
c HotoleanuHotoleanu, c Researcher  
f DuicaDuica, f Researcher  
A NicolescuNicolescu, A Researcher  
S GitmanGitman, S Researcher  

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