Document Details

Document Type : Article In Journal 
Document Title :
Bioprocess for semi-industrial production of immunomodulator polysaccharide Pleuran by Pleurotus ostreatus in submerged culture
Bioprocess for semi-industrial production of immunomodulator polysaccharide Pleuran by Pleurotus ostreatus in submerged culture
 
Document Language : English 
Abstract : Pleuran, is a polysaccharide belongs to glucan group which made of D-glucose moieties linked with (1 -> 3)- beta and (1 -> 6) -beta glycosidic linkages. The importance of this compound is based on its wide biotherapeutic application as immunomodulator and anticancer polysaccharide. This compound is produced only naturally using specific type of mushroom named Pleurotus ostreatus. Traditionally, this type of mushroom was cultivated using solid state cultivation system in green houses. Nowadays, submerged cultivation is considered as alternative cultivation strategy for mushroom based on its many advantages such as: short cultivation time, high yield, fully controlled cultivation condition, and fewer steps in polysaccharide extraction and purification compared to solid state fermentation. Thus, the present work was focused on the development of pleuran production process in semi-industrial scale using submerged cultivation system. At first, high yield production medium was selected followed by study on the kinetics of cell growth and EPS production in shake flasks. Second, cultivations were conducted in semi-industrial scale using in situ sterilizable 16-L stirred tank bioreactor under controlled and uncontrolled pH conditions. The results showed that bioreactor cultivation under controlled pH condition improved EPS production process and the maximal volumetric and specific pleuran produced in this study were 1.98 g/L and 0.445 g/g, respectively. 
ISSN : 0022-4456 
Journal Name : JOURNAL OF SCIENTIFIC & INDUSTRIAL RESEARCH 
Volume : 72 
Issue Number : 11 
Publishing Year : 1434 AH
2013 AD 
Article Type : Article 
Added Date : Tuesday, July 25, 2017 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
Parisa MaftounMaftoun, Parisa Researcher  
Roslinda MalekMalek, Roslinda Researcher  
Mahmoud Abdel-SadekAbdel-Sadek, Mahmoud Researcher  
Ramlan AzizAziz, Ramlan Researcher  
Hesham El EnshasyEl Enshasy, Hesham Researcher  

Files

File NameTypeDescription
 42258.pdf pdf 

Back To Researches Page