Document Details

Document Type : Article In Journal 
Document Title :
Preliminary characterization of a placental factor inhibiting breathing in fetal sheep.
Preliminary characterization of a placental factor inhibiting breathing in fetal sheep.
 
Subject : Neonatology 
Document Language : English 
Abstract : Previous studies have revealed a placental extract that inhibits breathing in fetal sheep. In the present study of 29 chronically instrumented sheep at 132±1 days of gestation, infusion of the 1-10 kDa extract inhibited breathing in 76% of the experiments whereas Krebs’ solution inhibited it in 24%. It retained this activity after 6 months of freezing, after lyophilization, and upon lowering the pH during purication from 8·0 to 4·0, but it inhibited breathing in only 35% when the pH was lowered to 2·0. A signicant dose-dependent effect was observed from a 16-fold dilution to a 4-fold concentration. Treatment of the extract with proteinase K or boiling reduced the activity to 30% or 26% inhibition, respectively. The activity was not adsorbed to an ion-exchange column at pH 7·0 or 8· 0, but it was at pH 9· 0 and it eluted with increasing NaCl concentrations. On a polyacrylamide gel the activity was eluted at a Kav of 0· 66 (82% inhibition), corresponding to between 2·5 and 4·5 kDa. These ndings suggest that a peptide produced by the placenta, with a molecular mass between 2· 5 and 4·5 kDa, inhibits fetal breathing 
ISSN : 1031-3613 
Journal Name : Reproduction, Fertility and Development 
Volume : 9 
Issue Number : 6 
Publishing Year : 1417 AH
1997 AD 
Article Type : Article 
Added Date : Monday, August 31, 2009 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
سعد الصاعديAl-Saedi, Saad ResearcherDoctorate 

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