Document Details Document Type : Article In Journal  Document Title : Characterization of the heat-released substance from rat ileal muscle Characterization of the heat-released substance from rat ileal muscle   Subject : Pharmacology  Document Language : English  Abstract : Previous studies have proposed that increasing the temperature around the rat isolated ileal muscles induces a heat-released substance (HRS) which causes a decrease in the contractile response. Therefore, we have studied various characteristics of this proposed HRS in animals exposed to heat-stress conditions. In these animals, the contraction of muscles in response to carbachol was decreased by increasing the temperature from 37 degrees C to 40 degrees C. Further, bathing the ileum in a conditioned medium prepared by incubation of the ileal muscle at 40 degrees C has shown less contraction at 40 degrees C than in presence of normal Krebs medium. In addition, conditioned mediums, prepared at 40 degrees C were run on SDS-polyacrylamide gel electrophoresis and have shown the presence of a distinct protein band at a m.w. region of 55 kDa. These results confirm our previous studies that increasing the temperature around the muscles induces the HRS that causes the decrease in the contractile response  ISSN : 1232-1966  Journal Name : Annals of Agricultural and Environmental Medicine  Volume : 6  Issue Number : 2  Publishing Year : 1419 AH 1999 AD  Article Type : Article  Added Date : Wednesday, March 30, 2011  Researchers Researcher Name (Arabic) Researcher Name (English) Researcher Type Dr Grade Email زهير دمنهوري Damanhouri, Zoheir Investigator Doctorate   أسامة طيب Tayeb, Osama Researcher Doctorate   Files File Name Type Description  29417.pdf pdf   Back To Researches Page