|
Document Type |
: |
Article In Journal |
|
Document Title |
: |
Quantification of periapical bone destruction in mice by microcomputed tomography. Quantification of periapical bone destruction in mice by microcomputed tomography. |
|
Document Language |
: |
English |
|
Abstract |
: |
Bacterial infections of the dental pulp result in tissue destruction and periapical bone
resorption. The availability of genetically engineered mouse strains is a major
advantage in the use of this model system for studies of periapical pathogenesis. The
main limitation of the mouse model is its small size, and the necessity for laborious
histologic analyses to quantify periapical bone destruction. In the present study, we
evaluated the use of a new technology, high-resolution micro-computed tomography
(micro-CT), for the rapid and non-invasive quantification of periapical bone
destruction. Periapical lesions were induced in the lower first molars of mice by
exposing the pulp to the oral environment. Mandibles were harvested on day 21 after
pulp exposure, and were subjected to micro-CT analysis, with 17-microm-thick
radiographic sections. Samples were then decalcified, embedded, and sectioned for
histology. The cross-sectional area of periapical lesions was determined by image
analysis of corresponding micro-CT and histologic sections. The results showed a
highly significant correlation between micro-CT and histology (p < 0.0001), with mean
differences of 4. 1% (range, 0.9 to 7.2%) between the two methods. The mean error
associated with image analysis was 4.9% for images obtained by both micro-CT and
histology. The variability of replicate (n = 5) independent micro-CT determinations was
3.4%, less than that associated with the image analysis error. These results demonstrate
that micro-CT imaging is a rapid, reproducible, and non-invasive method, that gives
results that are closely comparable with those obtained by histology. Micro-CT appears
to have utility for the accurate quantification of changes in bone architecture in small
biological specimens. |
|
ISSN |
: |
0 |
|
Journal Name |
: |
J Dent Res |
|
Volume |
: |
79 |
|
Issue Number |
: |
1 |
|
Publishing Year |
: |
2000 AH
2000 AD |
|
Article Type |
: |
Article |
|
Added Date |
: |
Monday, May 5, 2008 |
|
Researchers
| خالد بالطو | Balto, Khalid | Investigator | Doctorate | kbalto@kau.edu.sa |
|