Document Details

Document Type : Thesis 
Document Title :
INVESTIGATION OF THE EFFECT OF THYMOQUINON ALONE OR IN COMBINATION WITH CISPLATIN ON CELL GROWTH, CELL CYCLE AND APOPTOSIS OF HUMAN ORAL EPITHELIAL SQUAMOUS CELL CARCINOMA CELLS IN VITRO
دراسة تأثير ثيموكيونيون لوحده او مع السيسبلاستين على نمو وتطور الخلايا السرطانيه المغزليه الفميه
 
Subject : Faculty of Medicine > Medical BIOCHEMISTRY 
Document Language : Arabic 
Abstract : An alternative medicine used for thousands of years by using a natural product such as polyherbal preparations. These used without scientific verification to treat various diseases. Their uses depend on physician's experience over the centuries. Oral cancer although preventable, it has a low survival rate that has not been changed over the last few decades. Cisplatin is a commonly used chemotherapeutic drug for oral cancer treatment. Unfortunately, its long-term use is associated with a lot of side effects complications and drug resistance. In a trial to find a safe, affordable and effective treatment, we investigated the effects of Thymoquinone (T.Q) the active component of Nigella Sativa alone or in combination with cisplatin (CDDP) on cell growth and apoptosis in oral squamous carcinoma cell line. The research is a preclinical in vitro study using UMSCC-14C squamous carcinoma cell lines as well as Oral Squamous Epithelial cell (OEC). Cell viability was assessed using WST-1 assay after incubating cells with TQ ± cisplatin treatment at different concentrations and duration. Cell cycle progression and apoptosis were assessed by flow cytometry. Western blot was used to assess some key apoptotic Proteins ( p53, caspase-9 and Bcl-2). Our results demonstrate that TQ significantly reduced the cell viability of UM-SCC cell line, in a time and concentration-dependent manner. Also, the results revealed that Thymoquinone and/or Cisplatin have anti-proliferative property in UM-SCC cell lines. Also, TQ induced apoptosis in UM-SCC cell lines as indicated by up-regulation of p53 and caspase 9 and downregulation of Bcl-2. Interestingly, the combination of TQ with CDDP showed strong synergism in UMSCC-14C cell line. Thus, the findings may offer an alternative strategy for the development of potential antineoplastic therapies against squamous cell carcinoma. 
Supervisor : Dr.Ayman Zaky Elsamanoudy 
Thesis Type : Master Thesis 
Publishing Year : 1437 AH
2016 AD 
Added Date : Sunday, June 26, 2016 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
عمر مقبل العوفيAl Aufi, Omar MoqbelResearcherMaster 

Files

File NameTypeDescription
 39270.pdf pdf 

Back To Researches Page