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Document Type |
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Thesis |
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Document Title |
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Breast cancer سرطان الثدي |
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Subject |
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Faculty of Applied Medical Sciences |
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Document Language |
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Arabic |
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Abstract |
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Breast cancer (BC) is the most common invasive cancer in females worldwide. Despite the advances in clinical applications, laboratory and biomedical research, cancer remains a leading cause of mortality worldwide. The association between BC and obesity is still poorly understood. Adenosine mono phosphate–activated protein kinase (AMPK) is a metabolic regulator that promotes energy conservation and restoration when cells are exposed to nutrient stress. It is suggested to act as a link between obesity and BC and its activation is considered as a potential preventative and therapeutic target. The aim of this study was to investigate the possible association of obesity with AMPK by examining the correlation of p-AMPK immunohistochemical with obesity and the clinicopathological parameters in BC. Expression of the active form of AMPK, p-AMPK was evaluated in 138 primary BC and 27 matched non tumor-adjacent tissue samples using tissue microarrays and immunohistochemistry. Several studies have suggested a relation of obesity to menopausal status, in BC. Consistent with literature findings, our studies have shown that the prevalence of obesity and overweight was higher among patients aged 50 years and over, 46% and 39%, respectively comparing with 14% of normal weight. Association of p-AMPK with the pathological prognostic factors, was significant with hormone receptor phenotypes (p=0.0148). In particular ER+ PR+ HER2-phenotype is significantly higher in breast tumors with positive p-AMPK epithelium. Histotypes also were shown to be significantly associated with p-AMPK immunostaining, DCIS and ductal histotypes were more prevalent in positive p-AMPK tumor cases (p=0.0093). Furthermore, BMI weas significantly associated with p-AMPK immunostaining, and a BMI <30 kg/m2 was more prevalent in positive p-AMPK tumor cases (p-value=0.0381). In conclusion, our results showed slight decreased in p-AMPK expression in BC in comparison with control group. Expression of p-AMPK could be a useful marker for some types of BC (dependant on hormone receptor and histotypes). Furthermore, these results also suggest a down regulation of the active AMPK in BC obese patients. |
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Supervisor |
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Dr. Kalthoum Ali Al - Saqqaf |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1438 AH
2017 AD |
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Added Date |
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Wednesday, April 5, 2017 |
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Researchers
| روان فهد الغبان | Al - Ghabban, Rawan Fahd | Researcher | Master | |
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