Document Details

Document Type : Thesis 
Document Title :
Characterization of Gene Mutations in Lymphoma Cases
تحديد الطفرات الجينية في حالات سرطان الغدد اللمفاوية
 
Subject : Faculty of Applied Medical Sciences 
Document Language : Arabic 
Abstract : The lymphomas are a type of lymphoproliferative disorders that occur due to the over production of the lymphoid system cells such as lymphoreticular stem cell, lymphocytes, reticulum cells and histocytes. The primary classification of malignant lymphoma depending on histopathological diagnosis is Hodgkin’s lymphoma and Non-Hodgkin’s lymphoma. According to Saudi Tumor Registry Annual Reports, 2012 “NHL is the 3rd commonest malignancy in male and 6th commonest in female adult. In the other hands, HL is the 11th commonest cancer in male and 7th commonest in female adults”. This study aimed to survey the possible presence of cancer hotspots mutations in archival lymphoma cases from Saudi Arabia and to correlate the presence of such mutations with known clinicopathological parameters in order to test their use as potential biomarkers. The study consisted of analyzing 37 lymphoma cases collected during the period from 2007 to 2010 processed for sequencing on the Ion Torrent personal genome machine using the Ampliseq cancer hot spot panel V2 from Thermofisher. Eleven samples generated high quality sequencing yield that revealed interesting mutational pattern. The most commonly mutated genes in our series of samples were TP53 (100%), ATM (91%), EGFR (91%), KIT (82%), and SMAD4 (73%). Many other genes were mutated at a lesser frequency which could reflect a significant heterogeneity in the lymphoma pathogenicity. The failure to generate sequencing data from the remaining samples reflect the need for a review of the storage protocols of archival cancer tissues. 
Supervisor : Prof. Mamdooh A. Gari 
Thesis Type : Master Thesis 
Publishing Year : 1438 AH
2017 AD 
Co-Supervisor : Dr. Ashraf Dallol 
Added Date : Sunday, June 4, 2017 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
سماح عبدالعزيز مخلصMokhlis, Samah AbdulazizResearcherMaster 

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