Document Details

Document Type : Thesis 
Document Title :
Clinical and molecular epidemiological characteristics of carbapenem-resistant K. pneumoniae (CRKP) isolates in Asir-hospitals, Saudi Arabia
الخصائص السريرية والجزيئية الوبائية لبكتيريا الكليبسيلا الرئوية المقاومة لمضادات الكاربابينيمات والمعزولة من مستشفىيات منطقة عسير، المملكة العربية السعودية
 
Subject : Faculty of Applied Medical Sciences 
Document Language : Arabic 
Abstract : Background: The emergence of carbapenem-resistant Klebsiella pneumoniae (CRKP) isolates is becoming a global problem for many reasons: Firstly, these isolates are usually associated with high mortality rate particularly among critically ill patients. Secondly, treatment options of CRKP infections are limited. Thirdly, many carbapenemase-encoding genes are carried on plasmids and can be easily spread to related species (e.g. Escherichia coli). Finally, the K. pneumoniae carbapenemases (KPC) are difficult to detect routinely in the clinical microbiology laboratory. This study aims to determine the most common β-lactamases among CRKP in Southern region of Saudi Arabia as this province has not yet been investigated for the emergence of CRKP. Methods: A total of 54 K. pneumoniae isolates with reduced sensitivity to carbapenems were obtained from various clinical specimens of Asir province hospitals during the period from the end of April 2015 to September 2015. Minimum inhibitory concentrations (MICs) of imipenem and meropenem were confirmed using E-test. Phenotypic detection of KPC and MBLs resistance enzymes was determined using carbapenemase detection set disks that has been developed recently. Molecular detection the most common carbapenemase resistant genes (blaIMP, blaOXA-48, blaVIM, blaNDM and blaKPC) was performed using Multiplex-PCR. Results: About of 46% and 50% of all tested isolates (n=54) were resistance to imipenem and meropenem respectively by E-test. Fourty-four isolates were harboring blaOXA-48 gene, 4 isolates were carrying blaNDM and one isolate was positive for blaVIM. None of the isolates were positive for both blaIMP and blaKPC. Conclusion: Carbapenem-resistant K. pneumoniae (CRKP) isolates harboring bla OXA-48 and blaNDM are emerging in Southern Province of Saudi Arabia. Healthcare providers from both governmental and private sectors must work continuously together to monitor and control the dissemination of carbapenemase producing isolates. 
Supervisor : Dr. Ibrahim Ali Al- Zahrani 
Thesis Type : Master Thesis 
Publishing Year : 1438 AH
2017 AD 
Added Date : Tuesday, July 18, 2017 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
بندر عبدالله الزهرانيAlZahrani, Bnader AbdullahResearcherMaster 

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