Document Details

Document Type : Thesis 
Document Title :
Evaluation of TP53 Deletions in Patients with Different Hematological Malignancies using FISH technique
تقييم الحذف الجيني لجين TP53 لمرضى المصابين بأورام الدم الخبيثة باستخدام تقنية التهجين الوميضي الموضعي
 
Subject : Faculty of Applied Medical Sciences 
Document Language : Arabic 
Abstract : Deletion of TP53- a tumor suppressor gene located on the short arm of chromosome17 (band p13), was detected in patients with different hematological malignancies. However, the significance of TP53 deletions on different hematological malignancies was not clear in some types of leukemia. In addition to that, the utility of using high sensitive assay as a routine diagnostic assay for TP53 expression was also not strongly addressed. Therefore, we aimed in this research to investigate the deletion of TP53 gene in 21 bone marrow samples from patients with different hematological malignancies by using florescent in situ hybridization (FISH) technique. Also, we investigated the association of TP53 abnormality with the karyotype and the clinical phenotype of each sample. Our study showed that patients with hematological malignancies especially (ALL "highly expressed", AML, NHL, and MDS) associate with significant loss of TP53 gene. We also correlated the results with the cytogenetic finding and we found that TP53 deletion is more significant with complex karyotype. Using this assay allow us to determine the status of TP53 in leukemia patient which could aid in the diagnosis and prognosis of the disease. 
Supervisor : Dr. Hebah AL-Khatabi 
Thesis Type : Master Thesis 
Publishing Year : 1438 AH
2016 AD 
Co-Supervisor : Prof. Mohammed H AL-Qahtani 
Added Date : Tuesday, July 25, 2017 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
عبدالله إبراهيم مذكورMathkoor, Abdullah IbrahimResearcherMaster 

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