Document Details

Document Type : Thesis 
Document Title :
ALCOHOL DEHYROGENASE ( ADH ) ISOENZYME AND ALDEHYDE DEHDROGENASE ( ALDH) ACTIVITY IN SERA OF BREAST CANCER AND FEMALE GENITAL TRACT CANCER PATIENTS IN SAUDI ARABIA AND THEIR DIAGNOSTIC VALUE AS TUMOR MARKERS
نشاط إنزيم نازعة هيدروجين الأكحول و نازعة هيدروجين الالدهايد في مصل الدم لمرضى سرطان الثدي في المملكة العربية السعودية و قيمتهما التشخيصية كدلالات للورم
 
Subject : Faculty of Medicine 
Document Language : Arabic 
Abstract : Breast cancer (BC) is the most common type of cancer around the world; especially in women; threatening their lives, and causing a rise in the number of deaths if not diagnosed and treated early. Alcohol consumption is considered as one of the risk factors for BC, but the exact role of alcohol in BC progression is still unclear. The main enzymes that are responsible for ethanol metabolism are alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH), The metabolism of cancerous cells differs in many ways from healthy cells. Some metabolic changes that predispose to carcinogenesis are reported to result in changes in ADH and ALDH activity. The changed level of these enzymes in cancer cells may be reflected by the enzyme’s activity in the serum. Therefore, the activity of ADH and ALDH could be measured in sera of patients with breast cancer and could thus be helpful for diagnosing the cancer. The aim of this study is to determine the activity of total ADH and total ALDH in sera of breast cancer patients in Jeddah, Saudi Arabia and investigate their possible role as tumor markers. This study is performed in a case control design on breast cancer patients who are visiting the oncology clinics at King Abdul Aziz University Educational Hospital, Jeddah, Saudi Arabia. It is considered the first of its kind to be performed in Kingdom of Saudi Arabia (KSA). This is done by analyzed total ADH and total ALDH activities in 18 specimens of serum. 9 specimens are for controls which are healthy women and the other 9 specimens are from BC patients who matched the inclusion criteria of the research. Enzyme-linked immunosorbent assay (ELISA) technique was used to determine the activities of these enzymes in the samples. The activities of these enzymes in serum of BC patients reflect changes of their activities inside tumor cells which may seems to be released from cancer cells or from organs damaged by metastatic disease e.g. liver. In this study, we found that the activity of total ADH enzyme in BC patient’s serum is significantly higher than healthy controls serum (p=0.001). Also, the total activity of ALDH enzyme is significantly higher in patient’s serum than in healthy ones (p=0.000). These results suggest a potential significance of ADH and ALDH as a marker of BC. Fortunately, this will promise our future in KSA for early diagnosis and detection of the disease which is more likely to have a better prognosis and more successful treatment which may indirectly benefit worldwide health care. 
Supervisor : Dr. Fayza Fawaz Al Fayez 
Thesis Type : Master Thesis 
Publishing Year : 1439 AH
2017 AD 
Co-Supervisor : Dr. Mohamed Ahmed Hassanein 
Added Date : Tuesday, December 12, 2017 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
عهود محمد الشربجيAl-Sharbaji, Ohood MohammedResearcherMaster 

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