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Document Type |
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Thesis |
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Document Title |
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THE POSTPARTUM EFFECT OF GESTATIONAL DIABETES ON CIRCULATION LEVEL OF SIRTUIN تأثير ما بعد الولادة لسكري الحمل على مستوى سيرتين في الدورة الدموية |
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Subject |
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Faculty of Applied Medical Sciences |
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Document Language |
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Arabic |
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Abstract |
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Gestational diabetes mellitus characterized by hyperglycemia during pregnancy and usually disappears after delivery. However, some GDM women may continue to have diabetes type 2 diabetes (T2DM) after delivery due to unknown reasons.Increased circulating oxidative stress has been reported in GDM women and this can have an adverse effect on both mother and fetus such as the development of type 2 diabetes and cardiovascular diseases later in life. Among sirtuin (SIRT) family, SIRT1 and SIRT3 are NAD-dependent deacetylases have received more attention recently as cytoprotective and antioxidant. The aim of this study was to investigate whether alterations in circulating levels of sirtuins (SIRT1 & SIRT3) and superoxide dismutase 2 (SOD2) present in GDM postpartum women, women with previous healthy pregnancy and T2DM using real time PCR and ELISA respectively. Our results showed that SIRT1 increased at mRNA and protein levels in GDM postpartum patients compared with control group (P= 0.027, n=6 and P= 0.058435, n=6 respectively). The increase in SIRT1 gene expression was 1.8-fold compared with the control. However, there was no significant change in levels of SIRT1 mRNA in women with T2DM (P= 0.743, n=5). Interestingly, SIRT3 and SOD2 expression levels were decreased in GDM postpartum women compared with control (P= 0.044, n=6-7 and P= 0.046, n=7 respectively). Similarly, figures were found in women with T2DM showed a significant decreased in SIRT3 (P= 0.037, n=5) and a non-significant decreased in SOD2 (P= 0.854, n=5) relative to control group.
In conclusion, this study showed that there were alterations in the levels of SIRT1, SIRT3 and SOD2 in GDM postpartum women. Similar altered levels of SIRT3 and SOD2 observed in T2DM women suggested their possible roles in diabetes pathogenesis induced by increased oxidative stress. |
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Supervisor |
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Dr. Samar Ahmad Sultan |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1439 AH
2017 AD |
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Co-Supervisor |
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Dr. Kalthoom Ali Al-Sakkaf |
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Added Date |
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Wednesday, December 13, 2017 |
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Researchers
| ندا حسن الزهراني | Alzahrani, Nada Hasan | Researcher | Master | |
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