Document Details

Document Type : Thesis 
Document Title :
THE GENETIC DIVERSITY OF CARPABENEMASE-PRODUCING PSEUDOMONAS AERUGINOSA ISOLATED FROM MAKKAH HOSPITALS, SAUDI ARABIA
التنوع الجيني لبكتيريا الزائفة الزنجارية المنتجة لإنزيمات الكاربابينيميز والمعزولة من مستشفيات مكة المكرمة
 
Subject : Faculty of Applied Medical Sciences 
Document Language : Arabic 
Abstract : Background: The Islamic holy places in Makkah, the Western region of Saudi Arabia, are visited annually by more than six million Muslims from about 140 countries for Umrah and Pilgrim. This huge number of people can contribute in the spread of many bacterial pathogens and their clones in the whole Western region. There is no surveillance program or any information about the epidemiology and clonality of many bacterial pathogens including carbapenem-resistant Pseudomonas aeruginosa (CRP) in the Western region. Aims: The aim of this study was to identify the genetic diversity and the most common clones of CRP in Makkah hospitals. Methods: Fifty P. aeruginosa isolates that were resistant to carbapenems were collected from King Abduallah Medical City hospital (KAMC), in Makkah. Multilocus sequence typing (MLST) was used to identify the most common sequence types (STs) of CRP in Makkah. Results: Majority of CRP patients were female (51.5%) and the age of the patients ranged from 21 to 85 years old. Most of the patients were in Intensive Care Unit (ICU) and the isolates were mostly recovered from sputum (n=28). The MLST analysis showed that the isolates belonged to two clonal complexes (CC235and CC253), and 17 singletons. The most common ST identified was ST235 and ST654 with 6 isolates each. Eleven isolates showed MLST profiles which were not found in MLST database and may be assigned as new STs. Conclusion and recommendations: This study provided the first report about the most common clones of CRP in Makkah. The high level of genetic diversity among CRP isolates could be attributed to the fact that Makkah is visited yearly by millions of Muslims which may contribute to the spread of many CRP clones in this geographical region. The limited funds have prevented the collection of more CRP isolates from all Makkah hospitals. The findings suggest that in the Western region which affected by a huge number of visitors, surveillance program should be established to monitor the clonal dissemination of all multidrug-resistant (MDR) bacteria including CRP. 
Supervisor : Dr.Ibrahim Al-Zahrani 
Thesis Type : Master Thesis 
Publishing Year : 1439 AH
2018 AD 
Added Date : Thursday, March 29, 2018 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
بشائر بشائر الاحمديAl-Ahmadi, Bashaer ResearcherMaster 

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