Document Details
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Document Type |
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Thesis |
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Document Title |
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ADJUSTMENT MICROPROPAGATION PROTOCOLS FOR OLIVE USING PRE-COOLING AND GROWTH REGULATORS COMBINATIONS. إستخدام معاملات التبريد ومنظمات النمو لموائمةبروتوكولات للإكثار الدقيق للزيتون |
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Subject |
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Faculty of Meteorology, Environment and Arid Land Agriculture |
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Document Language |
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Arabic |
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Abstract |
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To set a definite micro-propagation protocol for olive has always remained a challenging task owe to its woody nature. In this study four olive cultivars from Aljouf region of Saudia Arabia (Arbosana, Arbequina, Koroneiki and Sorany) were evaluated for their optimized micropropagation protocol. In first phase four olive cultivars were evaluated in three factorial arrangement of RCBD, for shoot induction and proliferation protocol on olive media using different concentrations of growth regulators (zeatin and BAP), and levels of pre-cooling treatments (0, 24 and 48 hours). The in vitro performance of all the cultivars was assessed using a range of parameters including, morphological (length of primary shoot, percentage of induced shoots, number of leaves shoot-1, number of shoots explant-1), physiological (chlorophyll a, chlorophyll b, carotenoids, CO2 absorption), primary metabolites (mannitol, starch, glucose, fructose, sucrose, raffinose, proline) and secondary metabolites (alkaloids, flavonoids, tannins, phenols). Significant effect of interactions between, pre-cooling and hormones (P x H), and varieties and hormones (V x H) was noticed on the mean values of morphological, physiological and metabolic paratetrs. However, no effect of three way interaction among precooling, varieties and hormones (P x V x H) was reported. All cultivars indicated the maximum values of morphological, physiological and metabolic parameters for increasing pre-cooling treatments, and increasing concentration of hormones, zeatin and BAP. However, performance of 48 hrs pre-cooling, Arbosana and Arbequina cultivars, and zeatin hormone was noteworthy at all levels of evaluation. Furthermore, the interaction of 48 hrs pre-cooling with 2.5 mgL-1 zeatin exceed to BAP as revealed by the parameters included in the study. Likewise, SEM micrographs endorsed the higher affectivity of zeatin compared to BAP in accelerating the uptake of nutrients from olive medium as revealed by the EDX spectrum. Findings of the study present more reliable results for olive shoot induction and proliferation as it basis on wide range of parameters.
In vitro rhizogenesis is the last step determining the success of micropropagation of woody plants like olive. Therefore, second phase of current study has delineated a successful protocol of root induction in proliferated microshoots of four promising olive cultivars by using different levels of auxins (IBA and NAA) and dark period. The effects of these treatments were screened at morphological (rooting percentage, root length, number of roots per shoot and days of root initiation) and biochemical (primary and secondary metabolites) levels by conducting the comprehensive study using three factorial arrangement in RCBD. Significant effect of interactions between, dark treatment and hormones (D x H), and varieties and hormones (V x H) was reported only on metabolites (starch, fructose, sucrose, proline and alkaloids), however no effect was reported on mean values of root morphological parameters. No three way interaction among the treatment was reported. Moreover, SEM micrographs of developing roots were generated to get the better understanding about the role of hormones in root architect regulation. Significant impact of increasing levels of hormones was detected in improving the root parameters as well as in triggering the metabolic activities. Our evaluations on morphological and biochemical basis ratified the higher rooting effectiveness of olive media with 2.5 mg L-1supplementation of IBA and NAA; however comparative study marked IBA more promising as compared to NAA. This was proved by high mean values of morphological and biochemical parametrs for IBA as compared to NAA. In addition all cultivars showed noteworthy performance on both morphological and biochemical fronts at 2.5 mg L-1 concentration of hormones; while the performance of Arbosana and Arbequina was noteworthy. Both cultivars depicted the higher mean values of morphological and biochemical parameters as compared to Soarany and Koroneiki, for the same concentrations of hotmones. The dark treatment showed only exceptional performance in increasing the rooting percentage, while no promising impact was noticed for other morphological and metabolic parameters. However, comprehensive assessment revealed promising performance of IBA in mediating growth and metabolic activities as compared to NAA. |
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Supervisor |
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Dr. Bahget T. Hamooh |
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Thesis Type |
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Doctorate Thesis |
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Publishing Year |
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1439 AH
2018 AD |
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Added Date |
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Monday, April 16, 2018 |
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Researchers
| زاهد حسين شاه | Shah, Zahid Hussain | Researcher | Doctorate | |
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