Document Details

Document Type : Thesis 
Document Title :
THE OPTIMUM PRODUCTION AND CHARACTERIZATION OF PHYTASE ACTIVITY USING A NEW MICROBIAL STRAIN
الإنتاج الأمثل وخصائص انزيم الفايتيز باستخدام سلالة ميكروبية جديدة
 
Subject : Faculty of Sciences 
Document Language : Arabic 
Abstract : Phytase enzyme is the major enzyme applied in the animal feed industry, which releases the phosphate bound in the grain and thus diminishes the need for addition of inorganic phosphate to the feed. Phytase was developed for use in animal feed based on the assumption that the substitution of inorganic phosphate with phytase would offer a significant environmental advantage. Phytase producing bacteria were isolated from different sites in north Jeddah, Saudi Arabia on phytase screening media (PSM). The results showed that one of 18 tested isolates was the best phytase producer (B4), by measuring phytase activity according to measuring the amount of inorganic phosphate. The effect of growth conditions on phytase production was also studied, and the results showed that, the best condition for the highest amount of phytase production was in the presence of glucose as carbon source and yeast as nitrogen source. The best results were obtained at 55 ̊C for 48h and pH 7.5. This study focused on phytase enzyme improvement, which was partially purified by using the ammonium sulfate precipitation (60% saturation) and immobilizing the purified phytase enzyme in sodium alginate beads. The results revealed that the thermal stability of immobilized phytase was higher than free enzyme. The effect of metal ions Mg+2, Fe+3, Hg+2, Cu+2, Na+ and Ca+2 at a concentration 0.2 M used in the study showed inhibitory effect on immobilized more than the free phytase enzyme. These results highlighted the biochemical and technical advantages of immobilized phytase over the free enzyme. 
Supervisor : Prof. Enas Nabil Danial 
Thesis Type : Master Thesis 
Publishing Year : 1439 AH
2018 AD 
Co-Supervisor : Dr. Hanan Saeed Alnahdi 
Added Date : Monday, July 2, 2018 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
عهود دخيل الله مفوزMufwez, Ohood DakeelallahResearcherMaster 

Files

File NameTypeDescription
 43542.pdf pdf 

Back To Researches Page