Document Details
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Document Type |
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Thesis |
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Document Title |
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ANTIMICROBIAL SUSCEPTIBILITY ANALYSIS OF BACTERIAL COMMUNITY IN THE HOSPITAL ENVIRONMENT تحليل حساسية مضادات الميكروبات من المجتمع البكتيري في بيئة المستشفى |
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Subject |
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Faculty of Applied Medical Sciences |
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Document Language |
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Arabic |
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Abstract |
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Antimicrobial-resistance is one of the major health problem nowadays on the local, regional, and global levels which is rapidly escalating in all kinds of microorganisms among community and hospitalized patients. Transmission of nosocomial infection especially with antimicrobial-resistant bacteria limited the prescription options of physicians. In this study, a culturomic analysis was performed of the bacterial community from the environmental samples of King Abdulaziz University Hospital (KAUH). In total, 84 swabs and 15 air samples were collected mainly from frequently touched surfaces of different intensive care units (ICUs), wards and nursing stations of each location. The highest colony forming units found at door knobs and room sink. In total, 360 bacterial isolates were purified from the collected samples, and were identified by MALDI-TOF technique or 16S rRNA gene sequencing. The isolates belonged to the four phyla mainly comprised of Firmicutes (44.4%), followed by Actinobacteria (30.1%) and Proteobacteria (19.9%). In hospital microbiome of KAUH, total 36 different genera were identified in this study and were mainly comprised of; Staphylococcus (31.4%), Micrococcus (13.05%), Arthrobacter (11.7%), Bacillus (8.9%), Pseudomonas (8.3%), Klebsiella (3.33%), Acinetobacter (2.2%) and Stenotrophomonas (2.2%). The isolates were predominantly comprised of Staphylococcus species, Coagulase-negative Staphylococcus, Micrococcus species, Bacillus species, Pseudomonas species, Stenotrophomonas maltophilia, Kebsiella pneumoniae and several other Gram-positive and Gram-negative bacteria. Among pathogenic/opportunistic isolates, Staphylococcus epidermidis was commonly found in majority of the studied area. In the Gram-positive isolates highest resistance was observed against benzylpenicillin followed by azithromycin, erythromycin, ampicillin and co-trimoxazole among the tested antibiotics. In Gram-negative bacteria, highest resistance was observed against amoxicillin/clavulanic acid, co-trimoxazole, ciprofloxacin, chloramphenicol and ceftazidimie/clavulanate acid. In conclusion, majority of the isolate from the hospital microbiome of KAUH are of environmental origin including few pathogenic and opportunistic pathogenic bacteria were identified that were resistant to some antibiotics. Further studies are required to monitor the trend of environmental microbiome in the local hospitals. |
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Supervisor |
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Dr. Muhammad Yasir Noor Wali |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1440 AH
2018 AD |
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Added Date |
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Sunday, September 30, 2018 |
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Researchers
| عبدالله محمد سبحي | Subahi, Abdullah Mohammed | Researcher | Master | |
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