Document Details

Document Type : Thesis 
Document Title :
The Distribution of Duffy Blood Group Genotypes in the Kingdom of Saudi Arabia
توزيع النمط الوراثي لفصيلة الدم Duffyفي المملكة العربية السعودية
 
Subject : Faculty of Applied Medical Sciences 
Document Language : Arabic 
Abstract : Duffy blood group (Fy) genotyping became a topic of interest in transfusion medicine due to the complicated evolutionary history of human and population migration. Although some methods have been developed for FY polymorphism genotyping, not all of them have been sufficiently described. We demonstrate the genotyping of the major alleles of the Fy blood system and describe FY alleles and their frequencies among donors in two different cities of the western region of Saudi Arabia. Aim of the study: To determine the distribution of the FY blood group genotypes and allele (FY*A, FY*B, FY*null) to improve our standing of the genetic admixture of the Saudi population. Subjects and method: 349 DNA samples were collected from randomly selected donors from several hospitals in Jeddah, Saudi Arabia, as well as from two hospitals in Makkah, Saudi Arabia. The donors were 231 healthy male, 196 females. Genotyping of FY gene was performed using touch down PCR. DNA sequencing was conducted using primers to screen the following SNPs: T-33C, G125A, and T265C. Result: The analysis of the overall G125A genotype demonstrated a dominance of AA genotype in 64% of the donors, whereas the GA and GG genotypes were found in 27.1% and 8.9% of the donors respectively. Interestingly, 41% of the donors were homozygous for the GATA mutation T-33C that lead to silencing the FY gene, while for C256T only 5 donors were heterozygous and none was found to be homozygous. This study was compared to survey conducted in Turaba the result showed that the number of individuals expressing the duffy phenotypes in Turaba was 3 times higher than the donors in this study. Conversely, the null phenotypes were high in Jeddah and Makkah blood donors with 40.7% than in Turaba 18%. Conclusion and Recommendation: It’s the first report showing a genotypically FY blood group among the Saudi population in two cities of Makkah province. The gene admixture affects the Variation in the FY gene allele distribution in both regions. More studies should be done as it may affect blood transfusions in the future. 
Supervisor : Dr. Walid Bawazeer 
Thesis Type : Master Thesis 
Publishing Year : 1440 AH
2019 AD 
Added Date : Tuesday, February 5, 2019 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
عزوف عبدالكريم عاشورAshour, Azoof AbdulkareemResearcherMaster 

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