Document Details

Document Type : Thesis 
Document Title :
PHENOTYPIC AND GENOTYPIC CHARACTERIZATION OF EXTENDED SPECTRUM BETA-LACTAMASES IN ENTEROBACTERIACEAE CLINICAL STRAINS.
دراسة خصائص الأنماط الظاهرية و الجينية لسلالات البكتيريا المعزولة سريرياً من الفصيلة المعوية المنتجة لإنزيمات البيتالاكتاميز واسعة الطيف.
 
Subject : Faculty of Applied Medical Sciences 
Document Language : Arabic 
Abstract : Background: Extended-spectrum beta-lactamases (ESBLs) are the major cause of resistance to beta-lactam antibiotics such as penicillins, cephalosporins and monobactams. They are derived from genes for the narrow-spectrum beta-lactamases by mutations that alter the amino acid configuration around the enzyme active site. They are typically encoded by plasmids that can be exchanged readily between bacterial species. Aims: To determine the prevalence of ESBLs among Enterobacteriaceae urinary clinical isolates from patients in King Abdulaziz University Hospital (KAUH) and to identify important ESBL producing genes using molecular detection by multiplex polymerase chain reaction (PCR). Methods: The present prospective study was carried out during a period of four months between November 2015 and February 2016 in the Department of Clinical and Molecular Microbiology Laboratory at KAUH in Jeddah, Saudi Arabia. A total of 100 Gram-negative bacterial strains were isolated from urine specimens in different hospital units. Demographic information and antibiotics profile of all isolates were recorded. All isolates were subjected to phenotypic typing for detection of ESBLs production. ESBLs positive isolates were then tested for the presence of ESBLs producing genes by using genotypic typing. Results: In our study, 54 of Enterobacteriaceae strains were phenotypically confirmed ESBLs producers; predominantly E. coli (36) and K. pneumoniae (14). The gene that predominated was blaCTX-M (13) followed by blaTEM (8) and blaSHV (7). The antimicrobial sensitivity testing high showed resistance to ampicillin, cefalotin, cefazolin, ceftriaxone and trimethoprim/sulphonamides. Among them in E. coli was more resistant than K. pneumoniae. The entire bacterial isolates in this study were completely sensitive to imipenem and meropenem. Conclusion and Recommendations: Definitive identification of ESBLs genes is only possible by molecular detection methods. Phenotypic tests need to be evaluated periodically as their performance may change with the introduction of new enzymes. Also this study highlights the need to establish antimicrobial resistance surveillance for Enterobacteriaceae to regularly monitor the trends and new types of resistance mechanisms in clinical isolates. 
Supervisor : Dr. Abdulaziz Omer Bamarouf 
Thesis Type : Master Thesis 
Publishing Year : 1440 AH
2019 AD 
Added Date : Tuesday, April 9, 2019 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
عهود فؤاد منديليMandeli, Ohood FouadResearcherMaster 

Files

File NameTypeDescription
 44275.pdf pdf 

Back To Researches Page