Document Details

Document Type : Thesis 
Document Title :
Anti-fungal Properties of Four Medicinal Plant Extracts Against Vaginal Candida Infections
خصائص التأثير ضد الفطري لمستخلصات أربع نباتات طبية ضد الإصابات المهبلية بفطر الكانديدا
 
Subject : Faculty of Science 
Document Language : Arabic 
Abstract : The emergence resistance of fungi to antifungal agents lead to serious problems on human health. Candida spp that cause vaginal infections now is resistant to some classes of azole, amphotericin B, and echinocandin, and this phenomenon is an emerging problem all over the world. Azadirachta indica, Citrullus colocynthis, calotropis procera, and Moringa oleifera are a medicinal plant used in the Kingdom of Saudi Arabia in traditional medicine practice to cure disease in humans. Therefore, the aim of the present study is to evaluate the antifungal properties of four medicinal plant extracts Azadirachta indica, Citrullus colocynthis, calotropis procera, and Moringa oleifera against Candida albicans and non-albicans that caused vaginal candida infections. The leaves of all plants were extracted by 80% methanol and water, the evaluation of the antifungal properties was by broth microdilution assay (Minimum Inhibitory Concentration (MIC), Minimum Fungicidal Concentration (MFC), and agar well diffusion method with different concentrations. Moreover, the determine of the antifungal susceptibility of the isolates fungi by antifungal agents’ disk, E-Test strip Minimum Inhibitory Concentration (MIC), and identified by internal transcribed spacer (ITS), VITEK 2 system, and CHROM agar Candida spp. The method of agar well diffusion and broth microdilution with different concentrations showed significant antifungal activity against 6 strains representing 86 isolate of Candida spp that was resistant to five antifungal agents’ disk. The antifungal susceptibility showed 15 strains of Candida spp from 86 was resistance to all antifungal agents’ disk. This is the first time we evaluated plant leaf extract on 6 strains of Candida albicans and non-albicans completely resistance to five antifungal agents’ disk. The result of this study revealed that all the four medicinal plant have antifungal properties against resistance Candida spp especially Citrullus colocynthis and Azadirachta indica. However, among all plants, the leaf extract of Citrullus colocynthis was possessed the strongest antifungal activity and have a promising source as antifungal agents for the treatment of infections. 
Supervisor : Dr. Ahmed Mahmoud Mohamed Al-Hejin 
Thesis Type : Master Thesis 
Publishing Year : 1440 AH
2019 AD 
Added Date : Sunday, May 5, 2019 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
وائل عبد الرحمن الصبحيAlsubhi, Wael AbdulrahmanResearcherMaster 

Files

File NameTypeDescription
 44407.pdf pdf 

Back To Researches Page