Document Details

Document Type : Thesis 
Document Title :
THE CIRCULATING LEVEL OF SIRTUINS AND SUPEROXIDE DISMUTASES IN TYPE 2 DIABETES
مستوى بروتينات السيرتوين وإنزيمات الديسميوتيز المضادة للأكسدة لدى مرضى النوع السكري الثاني
 
Subject : Faculty of Applied Medical Sciences 
Document Language : Arabic 
Abstract : Background: Type 2 diabetes mellitus (T2D) is a multi-factorial metabolic disease characterized by chronic hyperglycemia with disturbances in the metabolism of carbohydrate, protein, and fat due to defects in either insulin action or insulin secretion, or both. Unfortunately, Saudi Arabia has a high incidence of this disease between the Middle East and North Africa according to the latest report of international diabetes federation. Hyperglycemia has been proposed to augment the release of reactive oxygen species (ROS) leading to oxidative stress. Consequently, adaptive cellular response results in neutralization of ROS through the antioxidant enzymes, for example, superoxide dismutase (SOD). Moreover, sirtuin1 (SIRT1) is located in the nucleus has been shown reduced oxidative stress through deacetylating the transcription factors leading to increased expression levels of antioxidant enzymes. Whereas, sirtuin3 (SIRT3) is located in mitochondria and has been shown reduced oxidative stress in diabetic endothelial cells via deacetylation and activation of SOD2. The aim: To evaluate systemic levels of SIRT1, SIRT3 and SOD2 in whole blood of patients who have type 2 diabetes and healthy subjects. Methods: We evaluated the circulating levels of sirtuins (SIRT1 and SIRT3) and antioxidant enzymes (SOD2) in whole blood by measuring expression levels of mRNA using real-time PCR, and SOD2 protein level in serum was measured by ELISA, in 37 T2D patients (21 male, 16 female) and 21 control subjects. Results: Our results showed both SIRT1 and SIRT3 mRNA expressions were significantly reduced in patients group compared to control subjects at p value < 0.05. SOD2 mRNA expression and protein levels were significantly increased in T2D at p value < 0.05 and &< 0.021 respectively. Conclusion: This study showed alteration of circulating levels of sirtuins and superoxide dismutase in T2D patients, which might be, at least, in part due to oxidative stress. 
Supervisor : Dr. Samar Sultan 
Thesis Type : Master Thesis 
Publishing Year : 1440 AH
2019 AD 
Added Date : Wednesday, July 24, 2019 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
عبدالله معلاء الخالديAl-Khaldi, Abdullah MuallaaResearcherMaster 

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