Document Details
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Document Type |
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Thesis |
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Document Title |
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Evaluation Clinical Usefulness of Mammaglobin-A as a Novel Breast Cancer Biomarker تقييم فائدة استخدام الماموجلوبين-أ كمؤشر حيوي جديد لسرطان الثدي |
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Subject |
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Faculty of Applied Medical Sciences |
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Document Language |
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Arabic |
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Abstract |
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Breast cancer (BC) is the most common cancer in women, like many other types of cancer, patients who are diagnosed and treated early may have a better prognosis. Despite the vast development in the field, there is no sensitive marker to detect BC at its early stage, through simple blood tests. Mammaglobin-A (h-MAM) has recently been suggested as a specific marker for BC. Our aim was to evaluate h-MAM as a potential biomarker for BC. The gene expression of h-MAM in blood and its serum level were investigated in 80 female patients, 63 were diagnosed as breast malignant and 17 non-malignant. The samples were collected at the time of BC diagnosis by mammogram and before starting the treatments. The expression of h-MAM mRNA was measured by qPCR, its serum levels were assayed by Enzyme Linked-Immuno-Sorbent Assay (ELISA), and CA15-3 were measured by Cobas 6000 analyzers. The results showed significant up-regulation in hMAM expression in patients with malignant compared with non-malignant (p<0.05). They have also shown that nearly 79% of the patients with malignancy demonstrated a highly significant increase of serum h-MAM levels compared with 25% in CA15-3; furthermore, all these 25% patients were actually included within the 79% that shows an increase of serum h-MAM. Moreover, comparison studies on patients' general variables and clinicopathological characteristics in the malignant group showed no significant correlation of the studied parameters with serum level of the h-MAM. On the other hand, increased expression of h-MAM were significantly associated with; tumor size, tumor grade, histotypes, and hormone receptor phenotypes i.e. ER, PR and HER2 expressions, and the p-values were (p=0.003), (p=0.019), (p=0.022), (p=0.018), (p=0.015), (p=0.03) respectively. In conclusion, our results demonstrate a higher sensitivity for h-MAM in BC patients and suggest potential usefulness for its measurement as BC tumor marker, over conventional markers currently in use in the clinical laboratory such as CA15-3. |
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Supervisor |
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Dr. Kalthoom Ali Al-Sakkaf |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1440 AH
2019 AD |
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Added Date |
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Wednesday, August 21, 2019 |
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Researchers
| حسن علي الشهري | Alshehri, Hassan Ali | Researcher | Master | |
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