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Document Type |
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Thesis |
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Document Title |
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ANALYSIS OF TP53 MUTATIONS IN OVARIAN CANCER PATIENTS OF WESTERN PROVINCE OF KSA تحليل طفرات TP53 لدى مريضات سرطان المبيض في المنطقة الغربية للمملكة العربية السعودية |
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Subject |
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Faculty of Applied Medical Sciences |
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Document Language |
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Arabic |
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Abstract |
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Analysis of TP53 mutations in ovarian cancer patients of western province of KSA Rania Bader Baabdullah Abstract Background: Ovarian cancer (OC) represents the highest causality of death amongst all gynecologic cancers, as it diagnosed at advanced-stages of disease. Further, escalation of multidrug resistance in OC has far compounded the outcome of treatment modalities. Thus improving early diagnosis and chemotherapeutic response prediction with high specificity, evaluation and validation of TP53 gene as a biomarker along with existing biomarkers will be a novel therapeutic approach to minimize the short comings with the use of single biomarker, which lack compatibility and reproducibility in some individuals. TP53, a tumor suppressor is one of the most common mutated gene in cancer, it regulates cell cycle and apoptosis in the cell. Aims: In this study, we have investigated the TP53 mutations of OC patients at exons (4,5,7 and 8) in western region of KSA. Methods: The study included 18 OC cases and 9 healthy control samples from King Abdul-Aziz University Hospital (KAU, Jeddah), polymerase chain reaction and Automated DNA sequencing were carried out to analyze TP53 gene mutations in Exon (4, 5,7 and 8.) Results: Our TP53 mutation analysis shows 56% missense mutation in exon 4 at codon 72, Notably, all the mutations of codon 72 detected in stage II and III with 60% stage III and 40% stage II. The 44% of the samples was wild type, 28% was heterozygous mutation Pro/Arg and 28% was homozygous mutation Arg/Arg. In Exon 7, 17% missense mutation were observed at hotspot R248. No mutations were identified in exon 5 and 8. Conclusion: The association between TP53 mutations and OC was observed in small percentage of patients in the Western region of Saudi Arabia. More analysis with a larger sample size is needed to understand the entire picture of OC and to establish the association between OC and the presence of mutations. |
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Supervisor |
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Dr. Ibsar Mirjal |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1441 AH
2019 AD |
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Added Date |
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Wednesday, September 25, 2019 |
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Researchers
| رانيه بدر با عبدالله | Baabdallah, Rania Bader | Researcher | Master | |
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