Document Details

Document Type : Thesis 
Document Title :
THE EFFECT OF ANTIOXIDANTS COMBINATION ON STORAGE LESION PARAMETERS IN STORED RED CELLS UNITS SUSPENDED IN SAG-M MEDIUM
مدى تأثير دمج مضادات الأكسدة على علامات آفة التخزين في أكياس الدم المخزنة العائمة في المادة الحافظة SAG-M
 
Subject : faculty of Applied Medical Sciences 
Document Language : Arabic 
Abstract : Introduction: Under hypothermic conditions, stored Red blood cells (RBCs) may develop morphological and metabolic changes known as ‘storage lesion’ which affects the quality of the transfused RBCs to patients in need. Hence, rejuvenation studies have been introduced to reduce storage lesion such as the use of antioxidants. Objective: Assess the effectiveness of melatonin mixed with vitamin C on stored RBCs units compared to untreated units. Method: Case-Control study was conducted by taking two different RBCs units from two healthy donors and dividing it separately into four units. Untreated RBCs unit was compared to units treated with melatonin, vitamin C, and antioxidant mixture of melatonin/vitamin C. All units were refrigerated at 4° C and samples from each unit were obtained on day 0, 15, and 30. Assessment was accomplished by measuring RBCs’ overall oxidation capacity and the level of oxidative stress. The assessment was carried out by measuring RBCs production of microvesicles (MVs), total GSH content, total antioxidant capacity (TAC), lipid peroxidation (MDA), haemolysis, pH and sodium levels. Results: By day 30, the combination of melatonin and vitamin C showed about 16% decrease in microvesicles production and approximately 1.5x improvement in GSH levels, but no significant impact on MDA levels. The TAC was increased by approximately 15% in comparison to untreated unit. Moreover, haemolysis levels were below the recommended level of 0.8%. The results obtained were normalised and then compared due to the small sample size and to eliminate any false impact. Conclusion: The effectiveness of standalone melatonin on stored RBCs was minimal. However, when melatonin was combined with vitamin C, the efficacy of melatonin as an antioxidant was improved and showed better results in comparison to untreated units. Thus, the combination of different antioxidants may be a promising approach to reduce storage lesion in stored RBCs units. 
Supervisor : Dr. Waleed Mohammed Bawazir 
Thesis Type : Master Thesis 
Publishing Year : 1441 AH
2020 AD 
Co-Supervisor : Dr. Mohamad Yassin Saka 
Added Date : Thursday, February 6, 2020 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
الهنوف عبد الرحمن المقبلAlmogbel, Alhanoof AbdulrahmaResearcherMaster 

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