Document Details

Document Type : Thesis 
Document Title :
Evaluating the Inhibitory Effects of Human Wharton’s Jelly Stem Cells on Acute Myeloid Leukemia Cell Line (K562) in vitro
تقييم الأثار المثبطة للخلايا الجذعية الهلامية على خط خلايا سرطان الدم الحاد الغير ليمفاوي في المختبر
 
Subject : Faculty of Applied Medical Sciences 
Document Language : Arabic 
Abstract : Acute myeloid leukemia (AML) is the most common form of leukemia and has high mortality with delayed treatment. Despite therapeutic advances, the cytotoxic side effects and poor prognosis in adults remain a great concern. Recently, human mesenchymal stem cells (MSCs) have been reported to have anticancer properties and is therefore considered to be a novel in cancer therapeutics. Of the existing stem cell types, the human umbilical cord derived Wharton’s jelly stem cells (hWJSCs) have several advantages, such as wide multipotency, hypoimmunogenicity, non-tumorigenic and anticancer properties. As such in the present thesis we evaluated the anticancer properties of hWJSCs against an AML cell line (K562) in vitro. Derived hWJSCs demonstrated the MSCs related CD markers expression using fluorescent activated cell sorting (FACS). The K562 cells treated with hWJSCs (co-culture), or its extracts namely, hWJSC-CM (100%) or hWJSC-CL (15 μg/mL) showed decrease in K562 cells proliferation and morphological changes leading to cell death at 24 h, 48 h and 72 h. AnnexinV-APC analysis showed the presence of apoptotic cells following treatment with hWJSCs co-culture (by 3.8% & 4.0%); hWJSC-CM (by 7.1% & 3.8%) and hWJSC-CL (by 9.2% & 4.5%) at both 24 h and 48 h respectively. Cell cycle analysis in general showed G2/M phase arrest following treatment with hWJSCs co-culture, hWJSC-CM and hWJSC-CL at both 24 h and 48 h. qRT-PCR showed downregulation of pro-apoptotic BAX genes compared anti-apoptotic genes (BCL2 and SURVIVIN) following treatment with hWJSCs and its extracts. Also, the cell cycle (CYCLIN A2 and CYCLIN E1) and inflammation related genes (IL-6, TNF),) were downregulated. The above studies indicate that hWJSCs and its extracts (hWJSC-CM and hWJSC-CL) inhibit K562 cell line in vitro and hope that identification of the specific molecules responsible for these inhibitory effects will pave way for novel therapeutics against acute myelogenous leukemia. 
Supervisor : Dr. Clamigam Gotman 
Thesis Type : Master Thesis 
Publishing Year : 1441 AH
2020 AD 
Added Date : Wednesday, June 3, 2020 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
منيرة عبد الحليم حويكمHuwaikem, Muneerah Abdul HalimResearcherMaster 

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