Document Details
|
Document Type |
: |
Thesis |
|
Document Title |
: |
ABCA1 C69T GENE POLYMORPHISM AND DISTURBANCES IN GLUCOSE REGULATION IN A SAUDI POPULATION التغيرات في الجين ABCA1 C69T بين السعوديين وعلاقته بتنظيم التحكم في الجلوكوز |
|
Subject |
: |
Faculty of medicine |
|
Document Language |
: |
Arabic |
|
Abstract |
: |
Introduction: The level of high-density lipoprotein cholesterol (HDL-C) is reported to be decreased in Type 2 diabetes mellitus (T2DM). Studies suggested that HDL-C enhance the uptake of glucose by cells, but its function is compromised in diabetes. ATP-binding cassette transporter 1 (ABCA1) has been reported to play an important role in the metabolism of HDL-C by helping in its formation. In pancreatic β-cells, the interaction of extracellular amphipathic apolipoproteins with ABCA1 leads to cholesterol efflux and insulin granules fusion and subsequent liberation of insulin hormone. Hence the loss of function of ABCA1 is expected to compromize insulin secretion. Indeed, studies suggested that mutations of ABCA1 is considered as a genetic risk factor for T2DM. Previous studies suggested that increase incident rate of T2DM is related to ABCA1C69T polymorphism. However, no studies in Saudi Arabia have been done to investigate the association between ABCA1 C69T gene polymorphism and low level of HDL-C in prediabetics. In this study, we estimated the prevalence of ABCA1 C69T (rs1800977) gene polymorphism in a sample of Saudi population, and the investigated the relationship of the variant with plasma lipid and glucose levels, as well as its association with impaired glucose tolerance.
Methodology: A Cross sectional Study was carried out on total of 651 Saudi adults recruited randomly from selected health centers in Jeddah. The genotyping analysis of ABCA1 C69T gene polymorphism was performed using a TaqMan SNP genotyping assay method.
Results: Genotypic distribution and allelic frequency was 11.4% Homozygous C/C, 43.8% Heterozygous C/C69T, and 44.8% Homozygous C69T/C69T. There was significant difference in the percentage of genotypic distribution among different ethnic group. Prevalence of dyslipidemia in males among CC was 55.3%, CT 62.5%, and TT was 57.3%, while in females among CC was 47.2%, CT 60.3%, and TT was 56.3%. Dysglycemic prevalence in males among CC was 18.4%, CT 31.9%, and TT was 23.6%, while in females among CC was 13.9%, CT 21.3%, and TT was 23%. No significant association between dyslipidemia and dysglyceima covariates with ABCA1 genotypes before and after adjustment for age or BMI and both.
Conclusion: ABCA1 genotypes differed significantly between people of different ethnic origin. No association with dyslipidemia and dysglycemia were found. The difference in ethnicity, and its effect on the frequency of different alleles might affect relative risk of dyslipidemia, and dysglycemia in different populations, and should be examined further to improve personalized medicine. |
|
Supervisor |
: |
Prof. Suhad M. Bahijri |
|
Thesis Type |
: |
Master Thesis |
|
Publishing Year |
: |
1441 AH
2020 AD |
|
Co-Supervisor |
: |
Dr. Ghada MA. Ajabnoor |
|
Added Date |
: |
Tuesday, June 23, 2020 |
|
Researchers
| وفاء سعد الرشيدي | Alrashidi, Wafa Saad | Researcher | Master | |
|
Back To Researches Page
|