Document Details
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Document Type |
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Thesis |
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Document Title |
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THE EFFECT OF HIGH GLUCOSE AND RESVERATROL ON CHONDROGENIC DIFFERENTIATION OF HUMAN WHARTON’S JELLY STEM CELLS تأثير ارتفاع تركيز الجلوكوز والريسفيراترول على التمايز الغضروفي للخلايا الجذعية الهلامية في وارتون البشرية |
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Subject |
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Faculty of Applied Medical Sciences |
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Document Language |
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Arabic |
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Abstract |
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Human Wharton’s Jelly Stem Cells (hWJSCs) isolated from umbilical cords has received more attention in the field of regenerative medicine including cartilage regeneration. Hyperglycemia affects stem cells proliferation and differentiation. Resveratrol (RSV) is a polyphenol compound with powerful anti-inflammatory property. This study investigated the effects of RSV, high glucose (HG) or both on chondrogenic differentiation, proliferation, and cytokine productions. The stemness of hWJSCs were characterized using flowcytometry. The cells were exposed to commercial differentiation medium with chondrogenic supplement and treated with HG, RSV or both for 14-21 days. During chondrogenesis, biological characteristics such as cell morphology and proliferation (WST-1 assay) were evaluated at day 4, 14 and 21. Toluidine blue staining was performed to detect the proteoglycans and glycosaminoglycans (GAG) deposition. On day 14, total RNA was isolated using RNeasy Mini Kit, and reverse transcribed to cDNA using ImProm-II™ Reverse Transcription System. RT-PCR was performed for the hyaline cartilage markers namely, COLI, COLII, COLX and SOX-9. Inflammation related cytokines namely IL-1A, IL-6, IL-8, G-CSF, and GM-CSF were detected in supernatant of differentiated cells collected at day 14 by using Multiplex Immunoassay. The proliferation test showed that HG treatment enhanced the growth of induced cells as observed on day 14 and 21. RSV treatment with or without HG decreased the growth of differentiated cell on day 4 while they enhanced their growth on days 14 and 21 relative to control. Compared with HG group, co-treatment of RSV with HG decreased the induced cells proliferation on days 14 and 21. The COLI, COLII, and COLX increased, while SOX-9 decreased in all treated groups compared to control, but the highest fold of collagen expression was observed in RSV plus HG group. IL-1A, IL-6, and IL-8 secretions were low in RSV with or without HG groups. The levels of G-CSF and GM-CSF were high in RSV plus HG group, while HG treatment reduced GM-CSF. In conclusion, under HG conditions, RSV promote chondrogenic differentiation as indicated by marked proteoglycan deposition and increased collagen expression may partially through cytokine regulations. |
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Supervisor |
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Dr. Samar Ahmad Sultan |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1441 AH
2020 AD |
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Added Date |
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Wednesday, June 24, 2020 |
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Researchers
| علي يحيى الألمعي | Alalmie, Ali Yahya | Researcher | Master | |
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