Document Details

Document Type : Thesis 
Document Title :
The Prevalence of Nasal Carriage of Community-Associated Methicillin-Resistant Staphylococcus aureus Among Healthy Adults in Jeddah Population
انتشار المكورات العنقودية الذهبية المقاومة للميثسيلين بين البالغين الأصحاء في مدينة جدة، المملكة العربية السعودية
 
Subject : Faculty of Applied Medical Sciences 
Document Language : Arabic 
Abstract : Introduction Community Associated Methicillin-resistant Staphylococcus aureus (CA-MRSA) is a major problematic pathogen. The morbidity associated with this pathogen has increased globally in recent years. In Saudi Arabia, very few studies have investigated the prevalence of CA-MRSA within the community. Aim of the study To determine the nasal carriage of CA-MRSA among healthy people in the city of Jeddah using different molecular and phenotypic methods. Materials and Methods Nasal swabs of community members who were present in different malls across Jeddah were collected in December 2018. Routine laboratory protocols were used for bacterial identification. CA-MRSA was confirmed using coagulase latex agglutination test and PCR targeting key genes (including coa, mecA, and PVL). SmaI -multiplex typing (SMT) was also used to determine the most common genotypes. Results A total of 485 nasal swabs were collected from healthy adults. Out of the total collected samples, 7.4% (36/485) participants were positive for CA-MRSA, and only two of them (0.4%) were positive for Panton-Valentine leukocidin (PVL) virulence gene. Based on SMT profiles, the genetic variations among the isolates were relatively high and most of the isolates exhibited SMT profiles that are more related to CA-MRSA. Only 9 isolates showed SMT profile that is identical to UK epidemic HA-MRSA-15 (EMRSA-15) profile. Three isolates recovered from one participant showed a similar SmaI-profile to well-known community-acquired strains [MSSA476 (CA-MSSA) and MW2(CA-MRSA)].Thirteen isolates did not show any SmaI-profiles or were not-typeable with SmaI-multiplex PCR. Conclusion This study clearly confirms the presence of CA-MRSA in Jeddah community. However, its pathogenic involvement at this stage cannot be detected. The lack of similar studies to compare this study findings might be considered as a limitation. More studies are inevitably needed to determine the common spreading clones of CA-MRSA in Jeddah as well as other major cities. To the best of our knowledge, this is the first study that focuses on CA-MRSA prevalence and genotyping in Saudi Arabia. 
Supervisor : Dr. Ibrahim Al-Zahrani 
Thesis Type : Master Thesis 
Publishing Year : 1442 AH
2020 AD 
Added Date : Sunday, February 28, 2021 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
محمد سعيد عبدهSaeed, Mohammed SaeedResearcherMaster 

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