Document Details

Document Type : Thesis 
Document Title :
THE EFFECT OF HIGH GLUCOSE ON THE EXPRESSION LEVELS OF CYTOKINES IN HUMAN WHARTON’S JELLY STEM CELLS
تأثير ارتفاع تركيز الجلوكوز على مستويات التعبير من السيتوكينات في خلايا وارتون جيلي البشرية
 
Subject : Faculty of Applied Medical Sciences 
Document Language : Arabic 
Abstract : Hyperglycemia induces tissue damage and stem cells act to restore tissue homeostasis. We evaluated the effects of high glucose (HG) on the Wharton’s jelly-derived stem cells (hWJSCs) morphology, proliferation, inflammatory cytokines and related gene expression. The hWJSCs were characterized for their stemness and divided into three groups as follows: I: Control (5 mM glucose); II: HG (28 mM); III: HG (41 mM). The cells were cultured for 24 h, 48 h, and 72 h and cell proliferation and morphology were determined. Inflammation related cytokines and associated gene expression were evaluated following 72 h exposure of the respective agents using Luminex multiplex assay and RT-PCR, respectively. Treatment with HG (Groups II and III) reduced the hWJSCs proliferation compared with control but did not affect the cell morphology. Secreted levels of IL-6 and IL-8 were reduced under high glucose conditions (28 and 41 mM). GM-CSF was reduced in the presence of HG (Groups II and III). IL-6 transcripts showed a similar trend as protein expression. IL-8 and GM-CSF mRNA was up-regulated in treatment groups (II-III). In conclusion, HG levels inhibited the proliferation of hWJSCs and caused alteration in pro-inflammatory cytokine levels. 
Supervisor : Dr. Samar Sultan 
Thesis Type : Master Thesis 
Publishing Year : 1442 AH
2021 AD 
Co-Supervisor : Prof. Abdulwahab Noorwali 
Added Date : Wednesday, May 26, 2021 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
أحمد محمد الزهرانيAlzahrani, Ahmed MohammedResearcherMaster 

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