Document Details

Document Type : Thesis 
Document Title :
MOLECULAR CHARACTERIZATION AND SUBTYPING OF STAPHYLOCOCCAL CASSETTE CHROMOSOME MEC TYPES IN METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS
التوصيف الجزيئي والتحت النوعي لـ)جين( بكتيريا المكورات العنقودية المقاومة للمضاد الحيوي (ميثيسيلين)
 
Subject : Faculty of Science 
Document Language : Arabic 
Abstract : Transmission of methicillin resistant Staphylococcus aureus (MRSA) occur inside the healthcare sector as nosocomial infection through healthcare workers or between admitted patients through skin, and hands, or by using the contaminated medical devices and dialysis system. Therefore, the current study was aimed to evaluate the genetic similarity of the MRSA recovered from inpatients and outpatients who visited a different health cares located in Jeddah A total of 200 (MRSA) strains were isolated from both inpatient and outpatient of which 105 (52.4%) were males and 95 (47.6%) were female from several popular hospitals located in Jeddah, during period of March 2018 to June 2019. The MRSA strains were isolated from respiratory tract 87 (43.5%), followed by skin and wound 66 (33.5%), urine 15 (7.3%), eye swab 11 (5.4%), blood 9 (4.7%), ear 5 (2.2%) and other tissues 7 (3.4%) respectively. Characterizations of the recovered strains had been carried out using both conventional and molecular methods. The results showed the most strains were identified as S. aureus and 92.5%. were detected as MRSA, while 7.5% were identified MSSA. PVL gene was detected in 32% of the samples including MRSA and MSSA. The typing and subtyping of SCCmec element revealed that the predominant type was type III (61.6%) followed by subtype IVa, IVb, IVd, and IVc respectively. Noteworthy, none of the isolates are showed type I, II and V among the mecA gene positive strains. The genetic relatedness among MRSA recovered from outpatients and inpatients clinics by RAPD-PCR revealed two main clusters without differentiation without differentiation between the outpatient and the inpatient strains. Fingerprinting of the examined strains with pulsed field gel electrophoresis revealed four major clusters, the first cluster contains three groups (16 strains all of them belonging to hospital acquired strains), these strains were isolated from patients suffered from respiratory and soft tissue infection and its of value to said that some of such strains were identical to each other with similarity index up to 100% indicating the same strain harbored in such hospitals. The second clusters contain two groups (12strains) also all of them recovered from in patients suffering from respiratory, soft tissue infections and urinary tract infections some of such strains also showed identical chromosomal patterns. Both the third and fourth clusters showed one group (6 strains and five strains respectively,) all of them recovered from outpatient’s clinics. 
Supervisor : Prof. Saleh Abdullah Ali Kabli 
Thesis Type : Doctorate Thesis 
Publishing Year : 1442 AH
2021 AD 
Co-Supervisor : Prof. Saleh Mohammed Saleh Al-Garni 
Added Date : Saturday, June 5, 2021 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
ياسر عبد الله الحجاجAl - Hajjaj, Yasser AbdullahResearcherDoctorate 

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