Document Details

Document Type : Thesis 
Document Title :
STUDYING THE ROLE OF MICRORNA IN FETAL ENDOTHELIAL DYSFUNCTION INDUCED BY GESTATIONAL DIABETES
دراسة دور المايكرو آر إن إيه في الخلل الوظيفي للخلايا البطانية للأجنة الناتجة عن سكري الحمل
 
Subject : Faculty of Applied Medical Sciences 
Document Language : Arabic 
Abstract : Hyperglycemia in gestational diabetes mellites (GDM) affects cells and tissues that involve epigenetic modifications of gene expression. In the fetus, epigenetic changes are believed to induce fetal metabolic programming of endothelial dysfunction, which increases the risk of cardiovascular disease in offspring during early adulthood. Therefore, there is a need to develop biomarker that could help in the detection of endothelial dysfunction early. One of the epigenetic mechanisms for fetal metabolic programming is miRNA regulation of gene expression. We aimed to determine whether GDM induce alterations in the expressions of 28 miRNAs related to endothelial dysfunction and cardiovascular diseases. MiRNAs were extracted from human umbilical vein endothelial cells (HUVECs) isolated from the umbilical cord of GDM (n=4), and control (n=4) women and cultured under normal glucose conditions up to 4 passages. Then differently expressed miRNAs were measured using q RT-PCR and TaqMan miRNA human Array plates. In HUVECs of GDM women, the expression of three miRNAs miR-140-3p (2-fold decrease; p =0.04), miR-1307-5p (2.5-fold decrease; p =0.015) and miR-342-3p (2-fold decrease; p =0.035) were significantly downregulated compered to control. Our data support the concept of epigenetic changes and showed that miRNAs expressions related to endothelial dysfunction and cardiovascular disease were altered in endothelial cells derived from women with GDM after maintained them in normal glucose conditions for several passages. 
Supervisor : Dr Samar Sultan 
Thesis Type : Master Thesis 
Publishing Year : 1443 AH
2022 AD 
Added Date : Thursday, January 19, 2023 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
ريهام حسن صبيحSabeeh, Reham HassanResearcherMaster 

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