Document Details
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Document Type |
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Thesis |
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Document Title |
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Molecular characterization of some plant growth-promoting bacteria isolated from the rhizosphere of halotolerant plants grown in Jeddah province التوصيف الجزيئي لبعض البكتريا المعززة لنمو النبات والمعزولة من المنطقة المحيطة بجذر النباتات المتحملة للملوحة النامية في محافظة جدة |
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Subject |
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Faculty of Science |
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Document Language |
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Arabic |
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Abstract |
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Background: Salinity is a major issue that causes significant crop productivity loss, and this stressed state makes crops more susceptible to disease caused by a variety of pathogens. In agriculture, plant growth-promoting rhizobacteria (PGPR) also known as plant growth-promoting bacteria (PGPB) that could produce ACC deaminase enzyme are an eco-friendly alternative to chemicals. For that reason, the current study was aimed to assess the presence of PGPR through the isolation and characterization of some ACC deaminase-producing rhizobacteria from the rhizosphere of halophytic plants grown in Jeddah Province.
Methods: In this study, three different halophyte plant species were collected from the coastal area of Thuwal, Jeddah, Saudi Arabia. The selective M9/ACC medium was used to isolate ACC deaminase-producing bacteria from plant roots. Fifteen isolates were selected and tested for their ability to promote the growth of faba bean seeds in vitro. The most effective isolates on the growth promotion were chosen for morphological, biochemical, and molecular identification.
Results: The three collected plant species were identified as Halopeplis perfoliata, Halocnemum strobilaceum, and Zygophyllum coccineum L. Huge number of ACC deaminase-producing were obtained. Four out of the fifteen isolates found to significantly promote the growth of faba bean seedlings compared to their untreated control. Finally, these isolates with plant growth promotion activity were identified as Bacillus haynessii for HS1, HS2, and HS3 isolates. While HS4 Isolate was identified as Bacillus lichenoformis.
Conclusion: In conclusion, this study achieved the isolation of ACC deaminase-producing bacteria with growth promotion activity. According to morphological, biochemical, and molecular identification methods they were found to be belonging to Bacillus genera. Therefore, the current findings suggest that the application of PGPR in agriculture is a supportable strategy for crop production increasing, especially in high salinity lands.
Keywords: Halocnemum strobilaceum, Halotolerant, Rhizosphere, PGPR , ACC |
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Supervisor |
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Prof. Dr. Nada Hassan AL-Qurashi |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1444 AH
2023 AD |
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Co-Supervisor |
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Prof. Dr. Sabah Mahmoud Mohamed Hassan |
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Added Date |
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Thursday, April 27, 2023 |
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Researchers
| بيان عبد الرحيم القصير | AL-Qusair, Bayan Abdulrahim | Researcher | Master | |
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