Document Details

Document Type : Article In Journal 
Document Title :
Purification and Characterization of two glycoproteins from the cattle tick Boophilus annulatus
تنقية وتعريف نوعين من الجليكوبروتينات من يرقات قراد البقر من نوع بؤفيلس أنيولاتس
 
Subject : Vaccine production 
Document Language : English 
Abstract : The present study was conducted to identify new target immunogenic molecules from the larval stage of the cattle tick, Boophilus annulatus (Acari: Ixodidae). Two specific larval glycoproteins (GLPs) were isolated by two-step affinity chromatography. The larval immunogens were first purified with CNBr-Sepharose coupled to rabbit anti-larval immunoglobulins, and the glycoproteins were then purified with Con-A Sepharose. These glycoproteins have molecular weights of approximately 32 and 15 kDa with isoelectric points between 6.8 and 7.2. Antibodies against the two GLPs, labeled I and II, were detected in the anti-whole tick, -whole larval, and -gut antigens through immunoblot analysis. These results suggest that these GLPs are good immunogens and can be useful in the vaccination of cattle against tick infestation 
ISSN : 1229-845X 
Journal Name : Journal of veterinary sceince 
Volume : 8 
Issue Number : 2 
Publishing Year : 1428 AH
2007 AD 
Article Type : Article 
Added Date : Saturday, January 15, 2011 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
عمرو السيد الحكيمEl Hakim, Amr El SayedResearcherDoctorateaselhakim@hotmail.com
ياسر عزت شاهينShahein, Yasser EzzatResearcherDoctorate 
أميرة كمال أبو العلاAbouellela, Ameera KamalResearcherDoctorate 

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